Fig. 4: Generation of aESCs and monkey from single embryos.

a Schematic diagram illustrating the experimental procedures. b Images of 2/4th and 3/8th embryos giving rise to newborn monkeys at 150 and 20 days after birth, respectively. Representative bright-field images of aESCs derived from 2/4th embryos and 3/8th embryos. Similar results were obtained in no less than three independently repeated experiments. c Representative IF images of aESCs from 2/4th and 3/8th embryos were stained for pluripotency markers OCT4, SOX2, and NANOG. Similar results were obtained in no less than three independently repeated experiments. d Histological analyses of teratomas formed by the aESCs revealed their potential to differentiate into all three germ layers in vivo. Similar results were obtained in no less than three independently repeated experiments. e, f Genetic analysis based on STRs examination shows that the nuclear DNA of the three-year-old and six-month-old monkey is consistent with that of aESCs but different from that of surrogate monkeys. The nuclear DNA of the newborn monkey and aESCs originated from their parents. All scale bar, 100 µm.