Fig. 5: Characterization of genetically matched aESCs, iPSCs, and ntESCs cultured on feeder and feeder-free layers.
From: Production of live monkeys and their genetically matched embryonic stem cells from single embryos
A Schematic diagram of the experiment. B Bright-field showing typical cell morphology of genetically matched aESCs, iPSCs, and ntESCs cultured on feeder and feeder-free layers. Similar results were obtained in no less than three independently repeated experiments. C Representative IF images of genetically matched aESCs, iPSCs, and ntESCs cultured on feeder and feeder-free layers and stained for pluripotency markers OCT4, SOX2, and NANOG. Similar results were obtained in no less than three independently repeated experiments. All scale bar, 100 µm.
