Fig. 4: Functional characterisation of ElSS-L1 and ElSS-L2 in N. benthamiana.
From: Evolution of linear triterpenoid biosynthesis within the Euphorbia genus
A Peplusol levels were quantified by LC-MS in N. benthamiana leaves infiltrated with: Empty Vector (EV), E. peplus Squalene Synthase-like1 (EpSS-L1, GenBank locus tag M5689_021805), E.lateriflora Squalene Synthase-like1 (ElSS-L1, GenBank accession PP978604), E. lateriflora Squalene Synthase-like2 (ElSS2, GenBank accession PP978605), with and without truncated A. thaliana HMG-CoA reductase (HMGRt, GenBank accession J04537.1). n.d.: not detectable, Error bars: SEM (n = 5 where n is the number of biological replicates). B Squalene levels were quantified by GC-MS in N. benthamiana leaves infiltrated with gene combinations as on panel A. n.d.: not detectable, Error bars: SEM (n = 5 where n is the number of biological replicates). Statistically significant (one-sided t-test) changes between control (EV) and candidate genes indicated by asterisks (**: p-value < 0.01). C 2,3-oxidosqualene levels were quantified by LC-MS in N. benthamiana leaves with gene combinations as on (A). Error bars: SEM (n = 4 or 5, where n is the number of biological replicates). Statistically significant (one-sided t-test) differences between control (EV) and candidate genes indicated by asterisks (**: p-value, < 0.01, *: p-value, < 0.05). Source data are provided as a Source Data file.
