Fig. 1: A sensor for pri-miRNA recognition and processing in C. elegans. | Nature Communications

Fig. 1: A sensor for pri-miRNA recognition and processing in C. elegans.

From: Interdependence of Pasha and Drosha for localization and function of the Microprocessor in C. elegans

Fig. 1

a pri-miR-58 sensor design. (i) In the presence of a functional pri-miRNA biogenesis pathway, the miR-58 hairpin is cleaved from the mCherry mRNA by the Microprocessor leading to the degradation of mCherry. (ii) If the sensor is not recognized and processed, mCherry is expressed. Created in BioRender. Montgomery, T. (2025) https://BioRender.com/e89g547. b mCherry fluorescence in animals containing the pri-miR-58 sensor or a control construct lacking pri-miR-58 sequence (mCherry control). Animals were treated with either control (empty L4440 vector) or pash-1 RNAi or were segregants for wild-type drsh-1 or the drsh-1 deletion allele drsh-1(ok369). Scale bars = 0.1 mm. At least three representative individuals were imaged for each condition. c Relative levels of mature miR-58 normalized to let-7 in the various strains indicated as determined by TaqMan qRT-PCR. Error bars are standard deviation (SD) from the mean. n = 3 biological replicates. Two-tailed, two-sample Student’s t-tests were used to calculate p-values for comparisons to wild-type. A Bonferroni correction for three comparisons was applied. Source data are provided as a Source Data file.

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