Fig. 4: PASH-1 WW domain-mutant proteins mislocalize to the cytoplasm. | Nature Communications

Fig. 4: PASH-1 WW domain-mutant proteins mislocalize to the cytoplasm.

From: Interdependence of Pasha and Drosha for localization and function of the Microprocessor in C. elegans

Fig. 4

a PASH-1::GFP expression in the adult germline and embryos (in utero). A wild-type animal is shown to highlight the non-specific background signal observed in germline tissue. The scale bars are 0.03 or 0.01 mm as indicated. At least three representative individuals for each strain were imaged. Imaging was repeated in three independent experiments with at least 10 individuals in total for each strain imaged. b mCherry::DRSH-1 expression in the adult germline. A wild-type animal is shown to highlight the lack of non-specific background signal observed in germline tissue. The scale bars are 0.03 or 0.01 mm as indicated. At least three representative individuals for each strain were imaged. c PASH-1::GFP and mCherry::DRSH-1 expression in 2-cell through 3-fold stage embryos. Strains lacking either PASH-1::GFP or mCherry::DRSH-1 are shown as controls for background fluorescence. The scale bars are 0.03 mm. At least three representative embryos for each stage were imaged. d PASH-1::GFP and mCherry::DRSH-1 expression in oocytes and 2, 4, and 16-cell embryos. A PASH-1::GFP embryo lacking mCherry::DRSH-1 is shown to highlight the lack of non-specific background signal observed for mCherry::DRSH-1 in embryos. Note both nuclear and cytoplasmic signal. The scale bars are 0.03 mm. At least two representative embryos or germlines were imaged for each strain. Imaging was repeated three times with at least 10 individuals for each strain in total imaged. e Relative mCherry fluorescence signal in the nucleus or cytoplasm of the embryos in (d). n = 2 (oocytes, 2-cell, and 16-cell) or 4 (4-cell) biological replicates. f, g PASH-1::GFP and PASH-1[G179R]::GFP (f) or PASH-1[W180A]::GFP (g) expression in the adult germline and embryos. Wild-type germlines and embryos are shown to highlight the non-specific background signal. The scale bars are 0.03 mm. At least fourteen representative embryos or germlines for each strain were imaged. Source data are provided as a Source Data file.

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