Fig. 5: PASH-1 and DRSH-1 promote each other’s nuclear localization. | Nature Communications

Fig. 5: PASH-1 and DRSH-1 promote each other’s nuclear localization.

From: Interdependence of Pasha and Drosha for localization and function of the Microprocessor in C. elegans

Fig. 5

a PASH-1::GFP, PASH-1[G179R]::GFP, PASH-1[W180A]::GFP, and mCherry::DRSH-1 expression in embryos. Wild-type embryos show the non-specific background signal. The scale bars are 0.03 mm. At least twenty embryos were imaged for each strain. b PASH-1::GFP and mCherry::DRSH-1 expression in embryos following control (L4440), pash-1, and drsh-1 RNAi. Wild-type embryos show the non-specific background signal. The scale bars are 0.03 mm. In the experiment shown, >100 animals for each condition were monitored, and 1 representative embryo was imaged. The experiment was repeated three times, with a total of at least 23 embryos imaged for each condition. c Relative pash-1 and drsh-1 mRNA levels following control (L4440), pash-1, and drsh-1 RNAi as determined by qRT-PCR. rpl-32 mRNA levels were used for normalization. Error bars are SD. n = 3 biological replicates. Two-tailed, two-sample Student’s t-tests were used to calculate p-values for comparisons to control RNAi. A Bonferroni correction for two comparisons was applied. d Relative PASH-1::GFP and mCherry::DRSH-1 protein levels after pash-1 or drsh-1 RNAi. Actin was used for normalization. Error bars are SD. One of 3 representative blot images is shown (see Source Data file). n = 3 biological replicates. Two-tailed, two-sample Student’s t-tests were used to calculate p-values for comparisons to control RNAi. A Bonferroni correction for two comparisons was applied. Bars are colored as in (c). Source data are provided as a Source Data file.

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