Fig. 5: A wave of macropinocytosis remodels the epithelium in response to external tissue compression.
From: Apical size reduction by macropinocytosis alleviates tissue crowding
a Representative image of an embryo injected with Cadherin-FP (cyan) and Lifeact-FP (red) before (left), during (middle), and after (right) a wave of macropinocytosis induced by tissue compression. Scale bar 30 μm. b Quantification of the wave of macropinocytic events (MP) upon compression (n = 80 scan areas from 32 embryos from 12 exp.; bars = mean, error bars = SD). c, f Representative images of embryos injected with Cadherin-FP (cyan/white) and Lifeact-FP (red) 5 min after compression in control (c) or Amiloride treated (f) embryos. Scale bar 10 μm. d, g Representation of tissue remodeling over 10 min using a temporal color code of control (d) or Amiloride (g) treated embryos. Scale bar 10 μm. e, h Quantification of tissue remodeling (i.e., overall change in cell apical area) (blue; left axis) and macropinocytotic events (red; right axis) in DMSO (e) and Amiloride treated (h) embryos upon compression (n = 3 exp.; bar = mean, error bars = SD). i Quantification of macropinocytotic events (red; left axis) and cell extrusion events (blue; right axis) in non-compressed (n = 9 embryos; MP p-value 5.00e-05, extrusion p-value = 0.0168) and compressed embryos in either DMSO (n = 32 embryos) or treated with amiloride (n = 13 embryos; two-sided unpaired t-tests; MP p-value = 1.31e-07, extrusion p-value = 0.0028) or LY294002 (n = 19 embryos; MP p-value = 4.19e-06, extrusion p-value = 0.0272). j Representative image of an embryo injected with Cadherin-FP (cyan) and Lifeact-FP (red) having both macropinocytotic events and cell extrusion (arrowhead) upon compression. Scale bar 30 μm. Source data are provided as a Source Data file.
