Fig. 1: Depletion of blood ApoM-S1P complex causes abnormality of the NSC pool in the SVZ.

a S1P concentration (n  =  5/group). b–d Representative immunofluorescence images and quantification of SOX2⁺GFAP⁺ cells (b), MASH1⁺ cells (c), and DCX⁺ cells (d) in the SVZ (n  =  6/group). Scale bars, 30 μm (enlarged, 15 μm) e Representative immunofluorescence images of SOX2⁺GFAP⁺BrdU⁺ cells (left) and quantification of SOX2⁺GFAP⁺BrdU⁺ cells and GFAP⁺BrdU⁺Ki67⁺ cells (right) in the SVZ (n  =  5/group). Scale bars, 10 μm. f Representative images (left), number (middle), and diameter (right) of primary neurospheres (n  =  7/group). Scale bars, 300 μm. g Representative immunofluorescence images and quantification of cleaved caspase-3⁺ and TUNEL⁺ cells in the SVZ (n  =  5/group). Scale bars, 50 μm. h mRNA levels of pro- and anti-apoptotic genes in the SVZ (WT, n = 6; Apom^-/-, n = 5). a–f Two-tailed student’s t test. All error bars indicate s.e.m. All data analysis was done at 4-month-old mice. Source data are provided as a Source Data file.