Fig. 7: Enhancement of blood ApoM-S1P complex ameliorates early events in APP/PS1 mice.
a S1P concentration (WT, n = 5; APP/PS1, n = 5; APP/PS1/ApomTG, n = 6; ApomTG, n = 4). b–d Quantification of SOX2+GFAP+ cells (b), MASH1+ cells (c), and DCX+ cells (d) in the SVZ (WT, n = 4; APP/PS1, n = 4; APP/PS1/ApomTG, n = 5; ApomTG, n = 4). e Quantification of SOX2+GFAP+BrdU+ cells in the SVZ (n = 5/group). f The number of primary neurospheres (n = 5/group). g Quantification of BrdU+ newly formed neurons in the GCL (left) and GL (right) in the OB (n = 5/group). h Quantification of CB+ cells, CR+ cells, TH+ cells in GL (n = 5/group). i, j The results of olfactory habituation–dishabituation test for both urine (i) and peanut butter (j) scents (WT, n = 11; APP/PS1, n = 13; APP/PS1/ApomTG, n = 12; ApomTG, n = 10). #P: WT vs APP/PS1, *P: APP/PS1 vs APP/PS1/ApomTG. k The latency to locate a buried food reward (WT, n = 12; APP/PS1, n = 13; APP/PS1/ApomTG, n = 11; ApomTG, n = 10). l Quantification of BB patch area in the walls of LV (n = 5/group). m Quantification of BB patch length/width ratios (n = 5/group). n Quantification of BB patch displacement (n = 5/group). o Analysis of angular distribution of BB patch angles in ependymal cells (WT, n = 289; APP/PS1, n = 314; APP/PS1/ApomTG, n = 311; ApomTG, n = 296; five animals/group). #P: WT vs APP/PS1, *P: APP/PS1 vs APP/PS1/ApomTG. p Quantification of CSF flow in the cerebral aqueduct (WT, n = 7; APP/PS1, n = 8; APP/PS1/ApomTG, n = 7; ApomTG n = 7). q Quantification of total ventricle volume (m), LV volume (WT, n = 7; APP/PS1, n = 8; APP/PS1/ApomTG, n = 7; ApomTG, n = 7). a Two-tailed student’s t test. b–h, k, l, n, p, q One-way analysis of variance, Tukey’s post hoc test. i, j Two-way ANOVA for repeated measures. m Contingency table test. o Two-sided Watson U2 test. All error bars indicate s.e.m. All data analysis was done at 4-month-old mice. Source data are provided as a Source Data file.
