Fig. 2: PLX3397 ablates microglia and CD206+ meningeal and perivascular macrophages.
From: Microglial cyclooxygenase-1 modulates cerebral capillary basal tone in vivo in mice
a Cartoon depicting the experimental approach (Created in BioRender. Eyo, U. (2025) https://BioRender.com/czwnlv4). b Representative composite 20X confocal image of somatosensory cortex in CX3CR1-eGFP mice for CD206 (magenta) and DAPI (grey), c the eGFP channel, and d the CD206 channel. Arrowheads denote the meningeal macrophages and arrows the perivascular macrophages. e The same as in b- except this composite representative image is from a mouse fed PLX3397 chow, f the eGFP channel, and g the CD206 channel. h The same as in b except this representative composite image is from a mouse that was fed control chow following PLX3397, i the eGFP channel, and j the CD206 channel. k Quantification showing averaged values from 42 fields of view/6 mice of total CD206 volume at baseline (mice fed control chow), mice fed PLX3397 (green), and repopulation (grey). Kruskal-Wallis test with Dunn’s multiple comparisons test. Baseline vs PLX, p < 0.0305; Baseline vs. WTDL, p < 0.0205; PLX vs. WTDL, p > 0.09999. l Volcano plot of total CD206 volume raw data points. m Quantification showing averaged values from 42 fields of view/6 mice of total perivascular macrophage volume at baseline (magenta), mice fed PLX3397 (green), and withdrawal (grey). One-way ANOVA with Tukey’s multiple comparisons test. Baseline vs. PLX, p < 0.0001; Baseline vs. WTDL, p < 0.0001; PLX vs. WTDL, p < 0.5167 n Volcano plot of total CD206 PVM volume raw data points. o Quantification showing averaged values from 42 fields of view/6 mice of total meningeal macrophage volume at baseline, mice fed PLX3397, and withdrawal. Kruskal-Wallis test with Dunn’s multiple comparisons test. Baseline vs. PLX, p < 0.0011; Baseline vs. WTDL, p < 0.0564; PLX vs. WTDL, p < 0.6880 p Volcano plot of raw data points. q Quantification showing averaged values from 42 fields of view/6 mice of total CX3CR1eGFP volume at baseline (mice fed control chow), mice fed PLX3397 (green), and withdrawal (grey). Kruskal–Wallis test with Dunn’s multiple comparisons test, Baseline vs. PLX, p < 0.0074; Baseline vs. WTDL, p > 0.9999; PLX vs. WTDL, p < 0.0148 r) Volcano plot of raw data points. Data presented as mean ± s.e.m. LUTs have been adjusted in shown images. Source data are provided as a source data file.
