Fig. 3: Effect of global ablation of myeloid cells on capillaries.

a Pie chart depicting the percentage of capillaries laden with CAM that increased or decreased in diameter by day 8 PLX. b Pie chart showing the percentage of capillaries laden with CAM that increased or decreased in diameter by day 8 repopulation (labeled WTDL). c (left) Bar graph comparing averaged diameter value reductions from 33 capillaries/4 mice. One-way ANOVA with Tukey’s multiple comparison test. Baseline vs. PLX, p < 0.0462; Baseline vs. WTDL, p < 0.6945; PLX vs. WTDL, p < 0.0376. c (right) Before-and-after line plot showing raw values used to construct c (left). d (left) Bar graph comparing averaged diameter value increases from 9 capillaries/4 mice. One-Way ANOVA with Tukey’s multiple comparison test. Baseline vs. PLX, p < 0.0006; Baseline vs. WTDL, p < 0.4547; PLX vs. WTDL, p < 0.5434. d (right) Before-and-after line plot showing raw values used to construct d (left). e 3D volumetric reconstruction where e is the composite image (top) with just the TRITC-Dextran channel (magenta) below. The white box in e indicates the measured CAM laden capillary portion. f 3D volumetric reconstructions showing the same capillary at day 8 PLX. g 3D volumetric reconstructions showing that same capillary at day 8 withdrawal (WTDL). The red dashed lines in e–g (bottom) represent the Vasometrics output used to generate the reported capillary diameter value. h–n The same as a–g, just for non-CAM laden capillaries. j (left) Bar graph comparing averaged diameter value reductions from 24 capillaries/4 mice. One-way ANOVA with Tukey’s multiple comparison test. Baseline vs. PLX, p < 0.0917; Baseline vs. WTDL, p < 0.9401; PLX vs. WTDL, p < 0.2001. j (right) Before-and-after line plot showing raw values used to construct j (left). k (left) Bar graph comparing averaged diameter value increases from 8 capillaries/4 mice. One-Way ANOVA with Tukey’s multiple comparison test. Baseline vs. PLX, p < 0.0518; Baseline vs. WTDL, p < 0.8209; PLX vs. WTDL, p < 0.7578. k (right) Before-and-after line plot showing raw values used to construct k (left). Data presented as mean ± s.e.m. LUTs have been adjusted in shown images. Source data are provided as a source data file.