Fig. 6: COX1 protein expression in microglia is higher than perivascular macrophages and astrocytes.

a 3D reconstruction of a 63X confocal image from somatosensory cortex in a CX3CR1-eGFP mouse. Myeloid cells are green, P2RY12 is blue, COX1 is white, and vessels are magenta. The arrow denotes a CX3CR1-eGFP^low perivascular macrophage and arrowhead denotes a CX3CR1-eGFP^high microglial cell. b 3D reconstruction of the same image in a but just showing the COX1 channel. c 3D reconstruction of the same image as in a but just showing the COX1 and P2RY12 channels. Note the arrow is in the same location as in a and confirms that the CX3CR1-eGFP myeloid cell there is P2RY12^negative. d 3D reconstruction of the same image as in a but just showing the eGFP channel. e 3D reconstruction of the same image as in a but just showing the P2RY12 channel. f 3D reconstruction of the same image as in a but just showing the lectin channel. g 3D reconstruction of a 63X confocal image from somatosensory cortex in an Aldh1l1-eGFP mouse. Astrocytes are in green, the water channel Aquaporin-4 labeling astrocyte endfeet is in cyan, and COX1 is in red. h 3D reconstruction of the same image as in g, but just the Aquaporin-4 channel. i 3D reconstruction of the same image as in g, but just the Aldh1l1-eGFP channel. j 3D reconstruction of the same image as in g, but just the COX1 channel. k Bar graph quantifying the percentage of total COX1 volume that is overlaid with CX3CR1-eGFP or Aldh1l1-eGFP volume. n = 8 fields of view across 3 mice for measurements in CX3CR1-eGFP mice and 8 fields of view across 4 mice for measurements in Aldh1l1-eGFP mice. Brown-Forsythe ANOVA test with Dunnett’s T3 multiple comparisons test. Multiplicity adjusted p values- MG vs. PVM, p < 0.0019; MG vs. Astrocytes, p < 0.0001; PVM vs. Astrocytes, p < 0.0688. l Violin plot showing the raw data used to generate the bar graph in (k). Data presented as mean ± s.e.m. LUTs have been adjusted in shown images. Source data are provided as a source data file.