Fig. 7: Genetic ablation of microglial COX1 results in significantly reduced capillary red blood cell flux and diameter.

a Cartoon depicting the experimental approach (Created in BioRender. Eyo, U. (2025) https://BioRender.com/h22a342). 3D reconstructions of a field-of-view at baseline (b) and 8 days after the last tamoxifen injection (f) in a TME119creERT2 Ptgs1fl/fl mouse. The capillary with red lines in the bottom white box represent the vasometrics output measurement for diameter. The top white box indicates the capillary from which a line scan was acquired, as shown in (c). c line scan at baseline (left) and day 8 after the last tamoxifen injection (right). 3D reconstructions of a field-of-view at baseline (d) and 8 days after the last tamoxifen injection (g) in a TME119creERT2 Ptgs1fl/wt mouse. The capillary with red lines in the top white box represent the vasometrics output measurement for diameter. The bottom right white box indicates the capillary from which a line scan was acquired, as shown in (e). e line scan at baseline (left) and day 8 after the last tamoxifen injection (right). h before-and-after plot showing the averaged capillary diameter values (left) and raw values (right) at baseline and day 8 in Ptgs1 fl/fl mice. n = 51 capillaries/3 mice. Two-tailed paired t-test, p < 0.0318. i Before-and-after plot showing the averaged capillary diameter values (left) and raw values (right) at baseline and day 8 in Ptgs1 fl/wt mice. n = 51 capillaries/3 mice. Two-tailed paired t-test, p < 0.1050. j before-and-after plots showing the averaged RBC flux values (left) and raw values (right) at baseline and day 8 in Ptgs1 fl/fl mice. n = 31 capillaries/3 mice. Two-tailed paired t-test, p < 0.0287. k before-and-after plots showing averaged values (left) and raw values (right) of RBC flux from control Ptgs1 fl/wt mice. n = 29 capillaries/3 mice. Two-tailed paired t-test, p < 0.7074. Source data are provided as a source data file.