Fig. 2: Differentially expressed genes in response to dietary iron supplementation in the gut and whole-body samples.

A, B Cluster analysis of 486 and 176 differentially expressed genes in the gut and whole body (WB), respectively. On the left, 10 clusters (C1–10) and 8 clusters (C1–8) are shown as circular heat maps. For each gene, the highest RPKM value was set to 100 (red), with the remaining seven time points normalized to this maximum. Numbers indicate hours after the dietary switch. The inner four circles depict iron-depleted conditions (“−iron”), and the outer four circles show iron-supplemented conditions (“+iron”). The graphs on the right show cluster profiles (C1–10 for gut and C1–8 for WB), each paired with a representative gene. All graphs show normalized RNA-Seq expression data. Time points (x-axis) indicate hours after the dietary switch. For each gene, the highest RPKM value was set to 100%, and the remaining time points were scaled proportionally. Each cluster profile represents the average relative expression (% of maximum) across all genes in that cluster at each time point. Error bars indicate the standard error at each time point. Asterisks denote significance based on a two-sided Student’s t test (*p < 0.05 and ***p < 0.001). Source data are provided in the accompanying source data file.