Fig. 3: Prussian blue staining of larval guts from selected RNAi lines.
From: New molecular components of high and low affinity iron import systems in Drosophila
A For each condition, 5–10 larval guts were stained with Prussian blue (ferrocyanide) and representative images are shown. Larvae were reared on iron-depleted (“− iron”, BPS-supplemented) or iron-supplemented (“+ iron”) diets. RNAi was driven by NP3084-Gal4, and NP3084 > w1118 animals served as controls. Blue staining indicates both the spatial distribution and relative levels of ferric iron accumulation. B Schematic summarizing the Prussian blue staining results shown in panel (A). Genes above the green line show higher expression under iron-depleted conditions, while those below the line are more highly expressed under iron-supplemented conditions. Gene names in green indicate known links to iron biology. AM anterior midgut, MM middle midgut, PM posterior midgut. Source data are provided in the accompanying source data file.
