Fig. 2: Flow guiding barrel transient improvements in onion epidermis.
From: Enhancing biolistic plant transformation and genome editing with a flow guiding barrel
a 22 ng of GFP-DNA and 10 μL of spermidine were bombarded into onion epidermis comparing the two delivery systems in transient cell transfection (n = 21 biological replicates for No Barrel, n = 8 for FGB). b 2.2 ng of GFP-DNA and 1 μL of spermidine were delivered with the FGB and compared with 22 ng of gfp-DNA and 10 μL of spermidine with conventional device (n = 23 biological replicates for No Barrel, n = 6 for FGB). c 25 μg of FITC-BSA and 1 μL of TransIT-2020 were delivered using both devices and compared by counting the number of FITC-BSA-positive onion cells (n = 5 biological replicates). d Transient Cas9-RNP editing in onion epidermis using both delivery systems verified by NGS (n = 9 biological replicates). The tracrRNA on the RNP-complex has red fluorescence, hence the greater red fluorescence is due to a more effective delivery of the RNP to the tissue. Box plots in (a) and (b) extend from the 25th to the 75th percentiles, with a box in the center representing the mean and whiskers extending from the minimum to the maximum values. Error bars represent the mean ± s.d. P values were calculated using a two-tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < .001. Scale bar = 1 cm. Source data are provided as a Source Data file.
