Fig. 5: Enhanced Shoot Apical Meristem (SAM) based transformation in wheat via FGB-aided biolistic delivery.
From: Enhancing biolistic plant transformation and genome editing with a flow guiding barrel
a Schematic illustration of the procedure for delivering DNA constructs to wheat SAM using biolistic delivery. Portions of this figure were created in BioRender. b Comparison between the conventional gene gun and the gene gun equipped with the FGB system. The FGB assembly features a modified stop screen, which differs in size from the conventional version. c Visualization of GFP expression under the two different conditions. The SAM region is outlined, with red arrows indicating GFP expression within SAM cells. d Quantification of GFP delivery efficiency using the SAM-based biolistic method and (e) Percentage of SAM cells exhibiting GFP fluorescence after bombardment. Error bars represent the mean ± s.d. (n = 3 independent experiments). P values were calculated using a two-tailed Student’s t test. *P < 0.05, **P < 0.01. The GFP fluorescence was detected 12 h after bombardment and embryos carrying 10 or more GFP signal spots were considered GFP-positive (d). The GFP fluorescence was detected 12 h after bombardment and embryos whose SAM (L1/L2/L3 layer) cells showing GFP signals were counted as positive (e).
