Table 1 Comprehensive summary of the experimental results evaluating SAM-based genome editing efficiency in wheat, comparing delivery with and without the FGB
From: Enhancing biolistic plant transformation and genome editing with a flow guiding barrel
Bombarded SAM | Shot | FGB (Y/N) | Flying distance | Psi | Positive T0 | T0 GE efficiency (%, mean ± sd) | Positive T1 | T1 GE efficiency (%, mean ± sd) | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Ex1 | Ex2 | Ex3 | Ex1 | Ex2 | Ex3 | ||||||||
Condition1 | 50 | 3 | N | 6 cm | 1350 | 1 | 3 | 2 | 4.0 ± 1.6 | 0 | 1 | 1 | 27.8 ± 20.8 |
Condition2 | 50 | 1 | N | 6 cm | 1350 | 0 | 0 | 0 | 0.0 ± 0.0 | 0 | 0 | 0 | 0.0 ± 0.0 |
Condition3 | 50 | 1 | Y | 6 cm | 650 | 4 | 5 | 4 | 8.7 ± 0.9 | 1 | 3 | 2 | 45.0 ± 14.7 |
Condition4 | 50 | 1 | Y | 9 cm | 650 | 4 | 6 | 4 | 9.3 ± 1.9 | 3 | 4 | 1 | 55.6 ± 21.9 |
Condition5 | 50 | 1 | Y | 6 cm | 900 | 2 | 1 | 3 | 4.0 ± 1.6 | 0 | 1 | 1 | 44.4 ± 41.6 |
