Table 2 Representative screening results of edited T0 wheat plants generated under different delivery conditions
From: Enhancing biolistic plant transformation and genome editing with a flow guiding barrel
| Â | T0 lines | Genome | Aligned sequence | Ratio (%) |
|---|---|---|---|---|
Ref | Â | GTCTTTGGGTGGTGAGGTCCGGCTGAATTCTCGTATTCAG | Â | |
Condition 1 | C1-2 | B | GTCTTTGGGTGGTGAGGTC-----------TCGTATTCAG | 3.72 |
Condition 3 | C3-2 | B | GTCTTTGGGTGGTGAGGTC-----------TCGTATTCAG | 2.69 |
C3-11 | B | GTCTTTGGGTG-------------------TCGTATTCAG | 3.76 | |
D | GTCTTTGGGTG-------------------TCGTATTCAG | 4.53 | ||
C3-13 | A | GTCTTTGGGTGGTGAGGTCCG---------TCGTATTCAG | 2.64 | |
Condition 4 | C4-5 | D | GTCTTTGGGTG-------------------TCGTATTCAG | 2.60 |
C4-8 | B | GTCTTTGGGTGGTGAGGTCCG-------TCTCGTATTCAG | 3.28 | |
C4-9 | D | GTCTTTGGGTG-------------------TCGTATTCAG | 3.73 | |
C4-10 | A | GTCTTTGGGTGGTGAGGTCCG---------TCGTATTCAG | 3.72 | |
Condition 5 | C5-3 | B | GTCTTTGGGTGGTGAGGTCCG--------CTCGTATTCAG | 3.46 |
