Fig. 7: Dynamics of the membrane protein DtpA.
From: Model-free photon analysis of diffusion-based single-molecule FRET experiments
a FRET efficiency histogram of DtpA in the detergent LMNG labeled at the cytoplasmic side of the two domains (inset). b Normalized correlation ratios of DtpA and averaged FRET trajectory of all bursts (inset). c FRET correlation function of DtpA computed from the data in (a). Solid black line is a fit with a sum of three exponential decays. Dashed blue line is the decay of the fastest component, green dashed line is the decay of the intermediate component, and red dashed line is the decay of the slowest component. The gray shaded area indicates the static heterogeneity (offset). d Residuals of the fits with multiple exponential decays. The number of exponentials is indicated.
