Fig. 1: RFC1 is expressed in the developing cerebellum.
From: RFC1 regulates the expansion of neural progenitors in the developing zebrafish cerebellum
A Whole-mount in situ hybridization for rfc1 shows specific, though not exclusive, expression in the developing cerebellum of zebrafish embryos at 1 and 2 days post-fertilization (dpf) (arrowheads; n ≥ 10 per stage). B–F RNAscope in situ hybridization detecting rfc1, ptf1a, and atoh1a transcripts at 1 dpf (B), 2 dpf (C), and 3 dpf (D); and rfc1, pvalb7, and neurod1 at 4 dpf (E) and 5 dpf (F). ot optic tectum, mhb midbrain-hindbrain boundary, URL upper rhombic lip, LRL lower rhombic lip, Va valvula cerebelli, CCe corpus cerebelli, LCa lobus caudalis cerebelli; n = 5 per stage. G–J Co-immunofluorescence for RFC1 (red) and either PAX6 (granule cell marker, green) or calbindin (Calb, Purkinje cell marker, green) in the developing mouse cerebellum at postnatal day P0 (G), P7 (H), P11 (I), and P60 (J). RFC1 is detected in Purkinje cell progenitors (PCp, arrows in G) but absent from granule cell progenitors (GCp, asterisks and arrowheads in G). From P11 onwards, RFC1 is observed in some migratory granule cells (mGC, arrowheads in I) and in Purkinje cells (PC, arrows in H, I). At P60 (J), RFC1 remains detectable in Purkinje cells (PC, arrows) and in ectopic granule cells located in the molecular layer (eGC, arrowheads). For each stage, images are representative of N = 3 animals for P0, P7, and P60, and N = 4 animals for P11, with n = 6 fields of view per animal. ML molecular layer, PCL Purkinje cell layer, IGL internal granular layer. Scale bars: 50 μm (B–F), 20 μm (G–J).
