Fig. 1: p53TAD2 is the primary binding site of FOXO4FH. | Nature Communications

Fig. 1: p53TAD2 is the primary binding site of FOXO4FH.

From: The disordered p53 transactivation domain is the target of FOXO4 and the senolytic compound FOXO4-DRI

Fig. 1

a Domain architecture of p53 and FOXO4. b 1H,15N HSQC spectrum of 100 µM 15N-labeled p531-312 in absence (black) and in presence of one stoichiometric (gray) or three stoichiometric (orange) equivalents of unlabeled FOXO4FH. c 1H,15N HSQC spectrum of 100 µM 15N-labeled p531–94 in absence (black) and in presence of one stoichiometric (gray) or two (orange) stoichiometric equivalents of unlabeled FOXO4FH, with corresponding CSPs. d 1H,15N HSQC spectrum of 100 µM 15N-labeled p53TAD2 in absence (black) and in presence of one stoichiometric (gray) or two (orange) stoichiometric equivalents of unlabeled FOXO4FH, with corresponding CSPs. e 1H,15N HSQC spectrum of 100 µM 15N-labeled FOXO4FH in absence (black) or in presence of one (gray) or two (blue) stoichiometric equivalents of unlabeled p531–94, with corresponding CSPs. f 1H,15N HSQC spectrum of 100 µM 15N-labeled FOXO4FH in absence (black) and in presence of one (gray) or two (blue) stoichiometric equivalents of unlabeled p53TAD2, with corresponding CSPs.

Back to article page