Fig. 3: Structural analysis of the FOXO4^FH – p53^TAD2 complex.

a Ensemble of ten lowest energy structural models of p53^TAD2 (orange) bound to FOXO4^FH (dark blue). Secondary structure elements and termini are indicated. b Ramachandran plot for residues 47–54 in p53^TAD2 along the different MD simulations of p53^TAD2 alone and in complex with FOXO4^FH, respectively. c Mean values of RMSD and RMSF of FOXO4^FH and p53^TAD2, respectively, in the FOXO4^FH-p53^TAD2 complex along the different MD simulations. The SD is shown as a shadow. d Structural details of the FOXO4^FH-p53^TAD2 interaction. e Complementary surface charge distribution of FOXO4^FH and key p53^TAD2 residues involved in the interaction (f) Comparison of the FOXO4^FH-p53^TAD2 with the crystal structure of FOXO4^FH (dark blue) bound to DNA (PDB: 3L2C) and the structure of the FOXO4^FH-FOXO4^CR3 complex⁴².