Fig. 1: Motor neuron transcriptome during postnatal remodeling.
From: Polyglutamylation of microtubules drives neuronal remodeling
a Strategy to isolate and sequence motor neuron-specific mRNA from ChAT-Cre mice crossbred to Rpl22HA. HA-tagged ribosomes (orange) and associated mRNA (cyan) were immunoprecipitated from spinal cords at different developmental time points (postnatal day, P5, 7, 9, 11, 14) and sequenced. b Confocal image stacks of longitudinal spinal cord sections of 8-week-old ChAT-Cre X Rpl22HA mice immunostained for choline acetyltransferase (ChAT, gray) and hemagglutinin (HA, orange), merged channels on the left (n = 3 mice). c Schematic illustration of tubulin post-translational modifications investigated in this study. Microtubules consist of alpha (α) and beta (β) tubulin dimers (gray), which may carry modifications in their luminal face (acetyl, red) or on their C-terminal tails (monoglutamate, pink; polyglutamate, magenta). Elongator TTLL glutamylases (turquoise) extend glutamyl side chains (polyglutamate, magenta); initiator TTLL glutamylases (gold) catalyze the addition of the first glutamate (monoglutamate, pink) to tubulin C-terminal tails; CCP deglutamylases (purple) remove glutamate residues. d–f Normalized mRNA read counts of (d) elongator TTLL glutamylases, (e) initiator TTLL glutamylases, and (f) CCP deglutamylases in motor neurons at postnatal day (P) 5, 7, 9, 11, 14 (n = 3 mice per age group). Graphs: Mean ± SEM. Scale bar, 25 µm.
