Fig. 4: CHIKV CD4+ T cell specific epitope megapool induces a robust response. | Nature Communications

Fig. 4: CHIKV CD4+ T cell specific epitope megapool induces a robust response.

From: Identification of immunogenic and cross-reactive chikungunya virus epitopes for CD4+ T cells in chronic chikungunya disease

Fig. 4

a Frequency of antigen-specific CD4+ T cells quantified by the AIM assay (OX40+CD137+) after 24 h stimulation with CHIKV epitope megapools (MP) consisting of epitopes from structural (S), non-structural (NS) and combined structural and non-structural (CHIKV_S + NS) proteins in 19 chronic CHIKV donors (chronic; blue dots) and seven CHIKV seronegative or uninfected donors (black dots; uninfected controls (UC)), with n representing the number of donors. % Positive refers to the percent of donors who are above the LOS (0.04), indicated by the dotted line. b Frequency of specific cytokine-producing cells (IFNγ, TNFα and IL-2) from the CHIKV-specific CD4+ T cells (AIM + OX40+CD40L+) after stimulation with the combined structural and non-structural CHIKV epitope MP (CHIKV_S + NS) in chronic CHIKV donors (n = 19). % Positive refers to the percent of donors that are above the LOS (0.003), indicated by the dotted line. c Frequency of CHIKV-specific AIM + CD4+ T cell memory subsets (AIM + OX40+CD137+) in chronic CHIKV donors (n = 19) post-stimulation with the CHIKV_S + NS epitope MP, based on the expression of CCR7 and CD45RA in AIM + CD4+ T cells as: T naïve (CCR7+CD45RA+), TCM (T central memory; CCR7+CD45RA-), TEM (T effector memory; CCR7-CD45RA-) and TEMRA (T effector memory re-expressing CD45RA; CCR7-CD45RA+). Each condition was tested once in individual experiments. Data are represented as geomean ± geometric SD (panels a and b) or as mean ± SD (panel c).

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