Fig. 2: Hierarchical clustering of the host top DEGs and gene set enrichment analysis.

A Heatmaps showing the hierarchical clustering of the top 50 DEGs of G. mellonella for four consecutive changes (Methods). Columns represent the biological replicates (3 replicates per time point), and rows represent the host gene symbols. Gene names are provided (Supplementary Data 5). Replicates are colored and grouped per time point. 0 h replicates are highlighted with yellow rectangles. Heatmap cells are colored by Z-score scale (−2 to 2). B Dotplots showing enriched pathways in biological processes and molecular functions in the host during infection. Each pair of plots shows enriched pathways of the top DEGs at each consecutive point. Each pathway is represented as a dot. The dots are colored by the p-adjusted value as computed by the gseGO function of the clusterProfiler R package (pvalueCutoff parameter of gseGO was set to <0.05). For testing differentially expressed genes, F-statistic, the associated P value, adj P value were corrected using Benjamini–Hochberg multiple testing correction (Statistics and reproducibility). The count represents the number of genes that belong to a given gene set, and the GeneRatio represents the count/setSize. setSize is the total number of genes in the gene set. Each pathway corresponds to a Gene Ontology (GO) term. Source data are available in the Gene Expression Omnibus (GEO) under accession number GSE213329.