Fig. 4: Host and pathogen transcriptomic changes before and after the development of Eumycetoma grain.

A The responses of host DEGs are shown in four patterns (Gradual, early, early long, and late response). Y-axis represents the Z-score of gene abundance, and the X-axis shows the time point and grain development stage. The gradual, early response and early long response show sub-patterns (down and up) shown as dashed or continuous lines, respectively. Full annotation of the genes in each response group in (Supplementary Data 6). B Expression of some of the host DEGs corresponding to the patterns in (A). Y-axis shows the count per million (CPM), and the X-axis shows the time point (n = 15, 3 biological replicates per time point). Data are presented as mean values ± SEM. C Transcriptomic response patterns of the pathogen DEGs. Only three patterns are detected. As in (A), Y and X axes represent the Z-score gene abundance and grain development stage, respectively. Full annotation of the genes in each response group is in Supplementary Data 8. D Expression of some of the pathogen DEGs corresponding to the patterns in (C) (n = 15, 3 biological replicates per time point). Data are presented as mean values ± SEM. We computed a two-way ANOVA test. Source data for (A, C) are available in the Gene Expression Omnibus (GEO) under accession number GSE213329. Source data for (B, D) are provided as a Source Data file.