Fig. 7: Validation of M. mycetomatis siderophore biosynthesis. | Nature Communications

Fig. 7: Validation of M. mycetomatis siderophore biosynthesis.

From: Iron regulatory pathways differentially expressed during Madurella mycetomatis grain development in Galleria mellonella

Fig. 7

A RP-HPLC analysis identified the time-dependent presence of three secreted metabolites (P1–P3) in untreated culture supernatants at A254 nm. P2 also showed weak A440 nm. B Post-ferration RP-HPLC revealed that P1 shifted to co-elute with P2 (increased A 254 nm and a 10-fold increase in 440 nm). C Spectrum scanning confirmed increased A440 + 20 nm, specifically following ferration for P2. D High-resolution LC-MS analysis of unferrated P1 (Rt 22.2 min) revealed a singly charged, putative siderophore with m/z = 855.2671 (inset) and a compound with m/z 822. E Since the species with m/z = 855.2671 disappeared upon Fe3+ addition, it represents a hitherto unidentified M. mycetomatis siderophore. Compound with m/z 822 is also evident in P2 (ferrated), and the nature of the species with m/z 769.3940 (inset) remains to be revealed. Source data are available via the PRIDE partner repository with the dataset identifier PXD063449.

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