Fig. 8: Effect of iron on the infection in G. mellonella larvae. | Nature Communications

Fig. 8: Effect of iron on the infection in G. mellonella larvae.

From: Iron regulatory pathways differentially expressed during Madurella mycetomatis grain development in Galleria mellonella

Fig. 8

A Toxicity of 332 µM EDDA, and 25 µM FeCl2 and FeCl3 in G. mellonella. B Survival of M. mycetomatis-infected larvae upon exposure to 332 µM EDDA, 25 µM FeCl2, and 25 µM FeCl3. C M. mycetomatis grain count in G. mellonella larvae upon exposure to 332 µM EDDA, 25 µM FeCl2, and 25 µM FeCl3. Data are presented as mean values ± SD, determined based on five biological replicates. D M. mycetomatis Grain size in G. mellonella larvae upon exposure to 332 µM EDDA, 25 µM FeCl2, and 25 µM FeCl3. Data are presented as mean values ± SD, determined based on five biological replicates. E Expression of G. mellonella genes related to iron regulation during infection upon exposure to 332 µM EDDA, 25 µM FeCl2, and 25 µM FeCl3 (n = 15, 3 biological replicates per time point). Data are presented as mean values ± SEM. We computed a two-way ANOVA test. F Expression of M. mycetomatis genes related to iron regulation during infection in G. mellonella larvae upon exposure to 332 µM EDDA, 25 µM FeCl2, and 25 µM FeCl3 (n = 15, 3 biological replicates per time point). Data are presented as mean values ± SEM. G Grocott stain of a M. mycetomatis grain in G. mellonella 72 h after infection and exposure to FeCl3. The arrows indicate the hyphae growing outside of the grain. The data shown are an example based on observations from five evaluated biological replicates. Source data for (A–F) are provided as a Source Data file.

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