Fig. 1: Single cell transcriptome of adipose tissue-derived endothelial cells.
From: Obesity drives depot-specific vascular remodeling in male white adipose tissue
a Representative confocal images showing CD31+ (orange) blood vessels and DAPI+ (cyan) nuclei in subcutaneous white adipose tissue (sWAT) or visceral WAT (vWAT) sections from mice fed either a control diet (CD) or high fat diet (HFD) for 8 weeks. b Quantification of blood vessel (CD31+) area in sWAT (CD n = 5, HFD n = 6 mice). c Quantification of blood vessel (CD31+) area in vWAT (CD n = 5, HFD n = 6 mice). d Comparison of vessel density in lean sWAT vs vWAT (sWAT n = 5, vWAT n = 5 mice). e Uniform manifold approximation and projection (UMAP) clustering of cells obtained from sWAT and vWAT from CD and HFD fed mice (dotted line represents clusters not discussed further). f Dot plot depicting top enriched marker genes in annotated clusters. The color intensity of each dot represents the level of marker expression, whereas the dot size reflects the percentage of endothelial cells (ECs) expressing the marker within the EC subcluster. g Demultiplexed UMAP of ECs in different depots in lean (CD) and obese mice (HFD). h Differential clustering of ECs in different depots and conditions. i Percentage of cells belonging to each annotated cluster under different condition and depots (CD n = 3, HFD n = 3 mice). Scale bar 200 µm. Data represents ± SEM, two-sided Welch’s t-test (b–d); two-way ANOVA with Tukey’s multiple comparison test (i). Source data are provided as a Source Data file.
