Fig. 4: Fenestrated endothelium in sWAT.
From: Obesity drives depot-specific vascular remodeling in male white adipose tissue
a Dot plot depicting enriched marker genes in fenestrated endothelial cells (ECs). b Quantitative real-time PCR detection of marker genes of fenestration in subcutaneous white adipose tissue (sWAT) ECs isolated from lean (control diet, CD n = 11 mice) and obese (high fat diet, HFD n = 11 mice) animals. c Representative orthogonal projections from whole mount staining for PLVAP (magenta) performed on sWAT from Esm1:Cre-ERT2 x mTmG reporter mice. Upon tamoxifen treatment, the reporter switches from tdtomato (yellow) to GFP (cyan). GFP+ blood vessels are also PLVAP+ (arrowheads). d Scanning electron micrograph showing fenestrae (see insets) in sWAT ECs in vitro. e Bioplanet annotated upregulated pathways in fenestrated ECs. f Gene ontology (GO) terms enriched in fenestrated ECs. g TTRUST transcription factor analysis (TF) of upstream regulators in fenestrated ECs. h Ingenuity pathway analysis (IPA) on differentially expressed marker genes in this cluster. Asterisk denotes VEGFA signaling. Orange and blue graphics represent predicted activation and inhibition, respectively. Straight lines and dotted lines represent direct or indirect interaction, respectively. i–k Violin plot depicting Vegf receptor levels in sWAT ECs in lean (CD) and obese (HFD) mice. l Quantitative real-time PCR detection of Vegf receptors and m Vegfa in sWAT ECs isolated from lean (CD n = 11 mice) and obese (HFD n = 11 mice) animals. Scale bar 100 µm. Data represents ± SEM, two-sided Welch’s t-test (b, l), Fisher’s exact test (e–g), two-sided Mann-Whitney test (m). Schematics created in BioRender. Hasan, S. (2025) https://BioRender.com/7rbmeru. Source data are provided as a Source Data file.
