Fig. 2: SG assembly in macrophages promotes AR progression.
From: Stress granule assembly impairs macrophage efferocytosis to aggravate allergic rhinitis in mice
G3bp1f/f and G3bp1mac-/- mice were administrated with OVA or HDM to induce AR symptoms as depicted in Supplementary Fig. 2e. a Times of sneezes and scratches in each mouse were counted in 15 min after last i.n. challenge from control, OVA and HDM group (n = 5, biological replicates). (Tukey’s HSD). b, c H&E and PAS staining of the nasal mucosa and lung tissues from AR mice. d Flow cytometry of eosinophils and T cells in the nasal mucosa of AR mice (n = 3, biological replicates). (Tukey’s HSD). e Flow cytometry of eosinophils and neutrophils in the NLF obtained from control and AR mice (n = 3, biological replicates). (Tukey’s HSD). f Flow cytometry of Mres in the nasal mucosa of control and AR mice (n = 3, biological replicates). (Tukey’s HSD). g mRNA expressions of indicated cytokines, Prg2 (proteoglycan 2, pro-eosinophil major basic protein), Epx (eosinophil peroxidase), and Mpo (myeloperoxidase) were detected by qRT-PCR in the nasal mucosa of AR mice (n = 3, biological replicates). (two-tailed unpaired Student’s t-test). h ELISA assay of IL-4, IL-5, and IL-13 in the NLF obtained from control and AR mice (n = 3, biological replicates). (Tukey’s HSD). i mRNA expressions of Il-10 were detected by qRT-PCR in NMMs of AR mice (n = 3, biological replicates). (Tukey’s HSD). j Serum OVA-specific IgE level in the control and AR mice (n = 3, biological replicates). (Tukey’s HSD). Data are shown as mean ± s.d. or photographs from one representative of three independent experiments.
