Fig. 3: NONO, SFPQ, and PSPC1 mediated telomerase recruitment is attenuated by mutation of the DBHS region.
From: NONO, SFPQ, and PSPC1 promote telomerase recruitment to the telomere
A Schematic of DBHS domain functions, mutations, or deletions. B Representative images of telomere (red), hTR (green), and coilin (purple) co-localizations in 293T 3′UTR NONO functional mutant rescue experiments (left panel). TTAGGG/hTR co-localizations are indicated by yellow arrows; hTR/coilin co-localizations are indicated by white asterisks. Scale bars are 5 μm. Tukey boxplots (median, interquartile range, and Tukey whiskers) of TTAGGG/hTR co-localizations (middle panel). Out of three experiments, n = 198 cells scored per treatment, n.s. = non-significant, *p = 0.0239, ****p < 0.0001, Kruskal-Wallis test. Violin plots of hTR/coilin co-localizations (right panel). Out of three experiments, n = 198 cells scored per treatment, n.s. = non-significant, ****p < 0.0001, Kruskal-Wallis test. C Representative images of telomere (red), hTR (green), and coilin (purple) co-localizations in 293T siSFPQ functional mutant rescue experiments (left panel). TTAGGG/hTR co-localizations are indicated by yellow arrows; hTR/coilin co-localizations are indicated by white asterisks. Scale bars are 5 μm. Tukey boxplots (median, interquartile range, and Tukey whiskers) of TTAGGG/hTR co-localizations (middle panel). Out of three experiments, n = 150 cells scored per treatment, n.s. = non-significant, ****p < 0.0001, Kruskal-Wallis test. Violin plots of hTR/coilin co-localizations (right panel). Out of three experiments, n = 150 cells scored per treatment, n.s. = non-significant, ****p < 0.0001, Kruskal-Wallis test. D Tukey boxplots (median, interquartile range, and Tukey whiskers) of TTAGGG/hTR co-localizations in 293T cells overexpressing PSPC1 functional mutants (left panel). Out of three experiments, n = 150 cells scored per treatment, n.s. = non-significant, *p = 0.0124, ****p < 0.0001, Kruskal-Wallis test. Violin plots of hTR/coilin co-localizations in 293T cells overexpressing PSPC1 functional mutants (right panel). Out of three experiments, n = 150 cells scored per treatment, n.s. = non-significant, ***p = 0.0004, ****p < 0.0001, Kruskal-Wallis test. E Representative images of telomere (red), hTR (green), and coilin (purple) co-localizations in 293T gPSPC1 cells overexpressing PSPC1 functional mutants (left panel). TTAGGG/hTR co-localizations are indicated by yellow arrows; hTR/coilin co-localizations are indicated by white asterisks. Scale bars are 5 μm. Tukey boxplots (median, interquartile range, and Tukey whiskers) of TTAGGG/hTR co-localizations (middle panel). Out of three experiments, n = 150 cells scored per treatment, n.s. = non-significant, ****p < 0.0001, Kruskal-Wallis test. Violin plots of hTR/coilin co-localizations (right panel). Out of three experiments, n = 150 cells scored per treatment, n.s. = non-significant, **p = 0.0033, ***p = 0.0005, ****p < 0.0001, Kruskal-Wallis test. Source data are provided as a Source data file.
