Fig. 2: ISA1 forms an extended dimer. | Nature Communications

Fig. 2: ISA1 forms an extended dimer.

From: Amylopectin branch trimming and biosynthesis elucidated by the rice isoamylase ISA1-ISA2 heterocomplex

Fig. 2

a Cryo-EM density map of the dimeric OsISA1. The two protomers are colored in slate gray and slate blue, respectively. b Structural model of dimeric ISA1, shown in cartoon representation in the same orientation as in (a). N and C termini are indicated. c Structural superposition of OsISA1 with CrISA1. OsISA1 protomers are colored in slate gray and slate blue, and CrISA1 protomers are in wheat and cyan, respectively. d Structural superposition of the dimerization domain of OsISA1 and CrISA1. e Structural alignment of the C-terminal dimerization domain of OsISA1 protomers A and B. The secondary structural elements are labeled. f Interaction interface of OsISA1 protomers. Key residues are labeled and shown in stick representation. g Enzymatic analysis of OsISA1 mutants using native gel analysis. At least three biological replicates were performed.

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