Fig. 1: Preparation and characterization of DiD-labeled liposomes (DiD-LIP) and lipid nanoparticles (DiD-LNP).
From: Evaluating cell cycle- and autophagy-associated cellular accumulation of lipid-based nanoparticles
a Schematic illustration of the synthesis of DiD-LIP (left, pink background) and DiD-LNP (right, blue background). For detailed synthesis procedures, please refer to the “Methods”. b Z-average and polydispersity index (PDI) of DiD-LIP and DiD-LNP. c Zeta potentials of DiD-LIP and DiD-LNP, with representative Cryo-TEM images (inserts) selected from 15 randomly chosen fields. Scale bars, 50 nm. d, e Z-average and PDI of DiD-LIP and DiD-LNP in DMEM containing 10% FBS stored at 37 °C for up to 24 h. Data in (b–e) were presented as mean ± SD (n = 3 independent biological replicates). Z-average and PDI among various time points were evaluated by one-way ANOVA, showing no statistically significant difference at α = 0.05 level: the Z-average of DiD-LIP showed F(4, 10) = 2.865, p = 0.081, η² = 0.534; the PDI of DiD-LIP showed F(4, 10) = 1.968, p = 0.176, η² = 0.440; the Z-average of DiD-LNP showed F(4, 10) = 3.482, p = 0.050, η² = 0.582; the PDI of DiD-LNP showed F(4, 10) = 0.698, p = 0.611, η² = 0.218. The level of significance was set at p < 0.05. Source data are provided as a Source Data file.
