Fig. 6: Assessment of correlations between nanoparticle accumulation and cell size.
From: Evaluating cell cycle- and autophagy-associated cellular accumulation of lipid-based nanoparticles
a Two-tailed Spearman correlation analyses showing the correlations between Total Fluorescence Intensity (TFI) and cell area for DiD-LIP (upper panel) and DiD-LNP (lower panel) in WT and ATG7 KO PIP-FUCCI cells at the two-dimensional (2D) level. For DiD-LIP: WT PIP-FUCCI (r = 0.685, p < 0.001, 95% CI [0.577, 0.775]); ATG7 KO PIP-FUCCI (r = 0.779, p < 0.001, [0.693, 0.844]). For DiD-LNP: WT PIP-FUCCI (r = 0.568, p < 0.001, [0.422, 0.687]); ATG7 KO PIP-FUCCI (r = 0.487, p < 0.001, [0.328, 0.628]). b Two-tailed Spearman correlation analyses showing the correlations between Total Fluorescence Intensity (TFI) and cell volume for DiD-LIP (upper panel) and DiD-LNP (lower panel) in WT and ATG7 KO PIP-FUCCI cells at the three-dimensional (3D) level. For DiD-LIP: WT PIP-FUCCI (r = 0.605, p < 0.001, [0.450, 0.728]); ATG7 KO PIP-FUCCI (r = 0.494, p < 0.001, [0.326, 0.646]). For DiD-LNP: WT PIP-FUCCI (r = 0.217, p = 0.020, [0.032, 0.394]); ATG7 KO PIP-FUCCI (r = 0.053, p = 0.588, [− 0.128, 0.234]). Cancer cells were respectively, incubated with DiD-labeled liposomes (DiD-LIP, at a dose equivalent to 5 μg/mL DOX) and lipid nanoparticles (DiD-LNP, at a dose equivalent to 6 μg/mL mRNA) for 2 h. The same volume of PBS was used as a control. All data were log10-transformed (n = 119, 113 cells for WT and ATG7 KO PIP-FUCCI treated with DiD-LIP; n = 114, 111 cells for WT and ATG7 KO PIP-FUCCI treated with DiD-LNP, respectively). The level of significance was set at p < 0.05, and the correlation strengths were determined using correlation coefficients (r). WT PIP-FUCCI, wild-type U2OS cells stably expressing PIP-FUCCI; ATG7 KO PIP-FUCCI, ATG7 knockout U2OS cells stably expressing PIP-FUCCI. Source data are provided as a Source Data file.
