Fig. 5: Stereocilia widening correlates with increased F-actin barbed and pointed levels in the shaft.
From: Myosin-dependent short actin filaments contribute to peripheral widening in developing stereocilia
a His-TMOD1 or His-CAPZ (magenta, grey) labeling in permeabilized IHCs 18 h after transfection with EGFP-actin (green). F-actin is stained with phalloidin (blue) to show stereocilia. Regions of interest are denoted by light blue dashed boxes and magnified to the right. For comparison, stereocilia shaft labeling of His-TMOD1 or His-CAPZ is indicated by blue arrows in untransfected cells and magenta arrows in EGFP-actin transfected cells. b–d Graphs showing the linear correlation of the EGFP-actin level in the stereocilia shaft with stereocilia width (b), His-TMOD1 shaft staining (c), and His-CAPZ shaft staining (d). Stereocilia are plotted as individual symbols and those from the same cell are represented by identical color and shape. Simple linear regression analysis was applied to the data. R-squared values for linear regressions from (b) to (d) are 0.66, 0.52, 0.55, respectively. Sample size (stereocilia, cells, cochleae): Width (592, 119, 22), His-TMOD1 (420, 84, 13), His-CAPZ (172, 35, 9). Scale bar represents 5 μm. Source data are provided as a Source Data file.
