Fig. 6: Polymerization-incompetent mutant actin disrupts the incorporation of wild-type actin at stereocilia tips.
From: Myosin-dependent short actin filaments contribute to peripheral widening in developing stereocilia
a IHCs 18 h after transfection with EGFP-actin (green) and mutant actins (magenta). Left panels: IHCs transfected with EGFP-actin alone or in combination with RFP-DVD-actin; Right panels: IHC transfected with EGFP-actin alone or in combination with RFP-AP-actin, adjacent to an untransfected IHC. White arrows denote the transfected constructs; selected regions are magnified in (b). Scale bar represents 5 μm. b Magnified insets from left to right: expression of EGFP-actin only, co-expression of EGFP-actin with RFP-DVD-actin or RFP-AP-actin. Scale bar represents 1 μm. c Line scans quantifying the fluorescence distribution of RFP-mutant actin relative to EGFP-actin from the co-transfected IHCs. The peak RFP level was set as 0 on x axis and the fluorescence intensity was normalized to the maximal fluorescence intensity of row 1. The line scan results were collected from −0.4 μm (above tips) to 0.8 μm (below tips) on x axis as described. The shadow lines represent individual stereocilia; the thick solid lines with error bars are the average level of all stereocilia with SD. Sample size (stereocilia, cells): DVD-actin (57, 19), AP-actin (48, 16). Source data are provided as a Source Data file.
