Fig. 2: ONT sequencing.

a Fraction of processed and unprocessed reads from total read counts in the indicated samples. Reads were classified as unprocessed if their ends did not fall entirely within ±20 nt of the annotated gene boundaries (see Supplementary Fig. 3a). b Proportions of individual unprocessed reads, containing mRNA and/or rRNA, as a percentage of the total unprocessed pool of reads for each sample. Reads representing less than 2% were pooled in the ‘other’ category. c Difference of fraction of individual unprocessed reads as a percentage of total reads in KO and Ctrl samples. Only junctions where the absolute difference exceeded 0.5% were visualised. Reads are indicated as most 5′ to 3′ mRNA or rRNA. Source data are provided as a Source Data file.