Fig. 5: The unprocessed mitochondrial rRNA cluster engage in early ribosome assembly.
From: The mitochondrial methylation potential gates mitoribosome assembly
Distribution of subunit levels of the (a) small or (b) large mitochondrial ribosome in fractions representing the mtSSU or monosome after SILAC labelling of Samc KO and control MEFs (n = 2). Right panel shows changes of individual subunits that are either increased (red) or decreased (blue) in the monosome fraction. Right panel Log fold change, and distribution of (a) mtSSU (b) mtLSU factors in the respective fractions. c Enrichment levels of mitochondrial RNA binding proteins and ribosome assembly proteins in ribosome gradient fractions in Samc KO and control MEFs after SILAC labelling (n = 2). d Northen blot analysis of ribosome gradient fractions from Samc KO and control MEFs (n = 1). Nucleotide length provided in grey (nts). Source data are provided as a Source Data file.
