Fig. 7: Formation of the PTC is inhibited in Samc KO MEFs.
From: The mitochondrial methylation potential gates mitoribosome assembly
a Close-up view of PTC maturation states. Panels show mtLSU assembly factors bound to part of rRNA helices and loops, contributing to the PTC, namely, h67-71 (pink), h80/P loop (gold), PTC loops (purple) and h92/A loop (blue). Lower panels show comparable states from published work while upper panels depict states A1-4, states B1, B2, C1 and D1 to depict the key stages of PTC maturation observed in current work. b 2D representation of domain V in mature mtLSU, human mtLSU intermediate (PDB ID 7O9M) and state D1. Interactions made by methyl transferases MRM2 and NSUN4 are highlighted. Residues methylated in mature mtLSU are highlighted (methylated as light green; unmethylated as grey). Dashed lines represent predicted stabilising interactions between methylated residues. Residue numbers are shown according to mouse genome numbering and the corresponding human numbering is indicated in brackets.
