Fig. 7: β-GalNAc Aβ9-21 (4b) rescues the cell toxicity of Aβ42, cognitive impairment, and neuron loss in APP mice.
a Cell viability assay performed with the CCK-8 method (n = 4). SH-SY5Y cells were incubated with different concentrations of Aβ and 4b for 24 h. The viability of untreated cells was set as 100%. b Schematic drawing of the schedule of the Morris water maze (MWM) test and tissue collection in C57BL/6 J mice treated with liposome vehicle (WT, wild-type, n = 9), APP/PS1 mice liposome vehicle (APP/PS1, n = 6), and APP/PS1 mice treated with 4b-liposomes (APP/PS1 + 4b, n = 8). i.v., intravenous injection. Created in BioRender. Yang, X. (2025) https://BioRender.com/55v38fv. c Representative swimming traces obtained during the probe trial. Red square, the starting point. The blue square represents the end point. Red circle, hidden platform. d Escape latency during the probe trial. e The number of times the mice passed through the area of the hidden platform during the probe trial. f The percentage of path length in the target quadrant of the hidden platform during the probe trial. g Nissl staining of the hippocampal region of the mice. Scale bar, 50 µm. h The number of neurons in the hippocampal region of the mice in (g) (n = 6). Statistical significance was assessed using two-side Student’s t-test. All values are presented as the means ± s.e.m.s. Source data are provided as a Source Data file.
