Fig. 1: Cryo-EM structure of the cpzA3H-VCBC complex. | Nature Communications

Fig. 1: Cryo-EM structure of the cpzA3H-VCBC complex.

From: HIV-1 vif mediates ubiquitination of the proximal protomer in the APOBEC3H dimer to induce degradation

Fig. 1

Top panel: 4 Å cryo-EM reconstruction (EM map), the atomic model, and arrangement of protomers (pink and black) of the cpzA3H-VCBC complex without symmetry applied (C1). Two molecules of cpzA3H (dark green and green) complexed with dsRNA (orange), Vif (dark blue), and CBFβ (dark grey) form the black protomer. The pink protomer consists of one molecule of cpzA3H (light green) bound to dsRNA (yellow), Vif (light blue), CBFβ (light grey), and EloBC (white). Representative cryo-EM densities of distal A3H, RNA, and the Zn-coordinating region of proximal A3H are shown in semi-transparent densities superposed with corresponding atomic models. Bottom panel: 3.6 Å cryo-EM reconstruction, the atomic model, and arrangement of protomers of the cpzA3H-VCBC complex with C2 symmetry applied. Representative cryo-EM densities of RNA and the Zn-coordinating region of proximal A3H are shown in semi-transparent densities superposed with corresponding atomic models. Both black and pink protomers contain identical component molecules: one molecule of cpzA3H (dark green in the black protomer, light green in the pink protomer), dsRNA (orange and yellow, respectively), Vif (dark blue and light blue, respectively), and CBFβ (dark grey and light grey, respectively).

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