Fig. 3: Across protomers RNA-Vif and cpzA3H-Vif interaction. | Nature Communications

Fig. 3: Across protomers RNA-Vif and cpzA3H-Vif interaction.

From: HIV-1 vif mediates ubiquitination of the proximal protomer in the APOBEC3H dimer to induce degradation

Fig. 3

a Atomic model of the cpzA3H-bound dsRNA from protomer 1 (green and yellow, respectively) and Vif from protomer 2 (pink). b Close-up view of the across-protomers interface between cpzA3H and Vif. The amino acids involved in these interfaces are represented in stick model format. c Close-up view of the interface between RNA and Vif amino acid residues across the protomers. d Schematic of the Vif amino acid residues involved in the RNA interactions across protomers. e Effect of amino acid substitutions at these RNA-Vif and cpzA3H-Vif interfaces on cpzA3H degradation. Individual Vif amino acid residues highlighted in (b) and (d) were substituted with alanines, and the degradation of FLAG-tagged cpzA3H was assessed (top). The intracellular levels of cpzA3H and Vif were probed 40 h post-transfection by Western blotting using anti-FLAG and anti-Vif mAbs. β-Tub was used as a loading control. The cpzA3H stability in the absence of Vif (“no”, lane 1) and presence of wild-type Vif (“WT”, lane 2) were used as controls. The results are representative from three independent experiments.

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