Fig. 5: Across protomers Vif-Vif interface. | Nature Communications

Fig. 5: Across protomers Vif-Vif interface.

From: HIV-1 vif mediates ubiquitination of the proximal protomer in the APOBEC3H dimer to induce degradation

Fig. 5

a Atomic model of the Vif-Vif interface formed between the protomers of the cpzA3H-VCBC complex. b Close-up view of the interface between Vif 1 (cyan) and Vif 2 (pink). The amino acids involved in this interface are represented in stick model format. c Schematic of the amino acid residues involved in Vif-Vif interactions. d Effect of amino acid substitutions at the Vif-Vif interface on cpzA3H degradation. The individual Vif amino acid residues highlighted in (c) were substituted with alanines, and the degradation of FLAG-tagged cpzA3H was assessed (top). The intracellular levels of cpzA3H and Vif were probed 40 h post-transfection by Western blotting using anti-FLAG and anti-Vif mAbs. An anti-ß tubulin polyclonal Abs (β-Tub) was used as a loading control. The cpzA3H stability in the absence of Vif (“no”, lane 1) and presence of wild-type Vif (“WT”, lane 2) were used as controls. The results are representative from three independent experiments.

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