Fig. 4: Artificial reactivation of a cocaine-recruited ensemble.
From: Cocaine-context memories are transcriptionally encoded in nucleus accumbens Arc ensembles
a Experimental design for CPP ensemble tagging and optogenetic-mediated reactivation Arc-CreERT2::Ai32 mice. 4-OHT 4-Hydroxytamoxifen, 1 P 1 Pairing, 5 P 5 Pairings. b Light stimulation of the tagged ensembles during CPP test. n = 9, 1-pairing; n = 8, 5-pairing. Two-way RM-ANOVA: interaction conditioning x light, F1,15 = 10.25, **p = 0.0059; main effect of conditioning, F1,15 = 0.8076, p = 0.3830; main effect of light, F1,15 = 0.3439, p = 0.5663. c ArcCreERT2::Ai32 mice were injected i.p. with 4-OHT and cocaine in their homecage and the tagged ensemble was optogenetically reactivated during the test session. Light stimulation of the homecage ensemble during CPP test. n = 8. Two-tailed paired t-test, t16 = 0.054, p = 0.9576. Bar and line graphs are expressed as means ± SEM. Source data are provided as a Source Data File. Created in BioRender. Parise, E. (2025) https://BioRender.com/voi7l54.
