Fig. 2: In vivo angiotensin II promotes the CD8+ T cells acquisition of effector functions and homing to vasculature through PI3Kγ.
a Immunohistochemistry of CD3+ (magenta) and B220+ (green) lymphocytes to analyze the white pulp area in the spleen of PI3Kγ+/+, PI3Kγ−/− and PI3KγKD/KD mice after in vivo administration of angiotensin II or vehicle as control. Nuclei are visualized in blue by DAPI. Images were acquired by confocal microscopy at 10X magnification (scale bar = 100 µm). b Quantitative analysis of the white pulp area marked by CD3+ T cells. n = 6 PI3Kγ+/+ Veh, n = 6 PI3Kγ+/+ AngII, n = 5 PI3Kγ−/− Veh, n = 7 PI3Kγ−/− AngII; n = 7 PI3KγKD/KD Veh and n = 5 PI3KγKD/KD AngII mice. c Analysis of the myogenic tone of mesenteric resistance arteries (MRA) dissected from naïve WT mice ex vivo co-cultured with CD8+ T cells purified from the spleen of PI3Kγ+/+ and PI3Kγ−/− mice stimulated in vivo for 3 days with angiotensin II or vehicle. n = 4 CD8Veh; n = 5 CD8AngII-PI3Kγ+/+ and n = 5 CD8AngII-PI3Kγ−/−. Representative plots of flow cytometry (d) and quantitative analysis (e–h) of total CD8+ T cells (e), naïve (f), effector (g) and resident memory (h) CD8+ T cells in kidneys dissected from PI3Kγ+/+, PI3Kγ−/− and PI3KγKD/KD mice after in vivo administration of angiotensin II or vehicle for 28 days. n = 5 PI3Kγ+/+ Veh, n = 7 PI3Kγ+/+ AngII, n = 6 PI3Kγ−/− Veh, n = 8 PI3Kγ−/− AngII, n = 6 PI3KγKD/KD Veh and n = 7 PI3KγKD/KD AngII. All data are expressed as mean ± SEM. Statistical analysis was performed by Two-way ANOVA, followed by Tukey’s correction for multiple comparisons in b, e, f, g, h; One-way ANOVA for repeated measures, followed by Tukey’s correction for multiple comparisons in c. N represents biologically independent samples. Source data are provided as a Source Data file. Schematics in a, c, d were created in BioRender. Perrotta M (2025) https://BioRender.com/1f77gjq, https://BioRender.com/fbmd4r3, https://BioRender.com/g0be7ts.
