Fig. 3: Citrullinated histone H3 at the HIV-1 provirus stimulated latency reversal.

a H3cit signal at a HIV 5´ LTR locus (HXB2 coordinates: 522-643) in 5A8 cells, measured by ChIP-qPCR (n = 6 for H3, H3cit, IgG; n = 3 for H3cit8, H3cit17). Exact p-values were calculated with two-sided unpaired Student's t tests, with correction for multiple testing by two-stage step-up (Benjamini, Krieger and Yekutieli). b H3 signal at the HIV 5´ LTR locus in 5A8 cells, measured by ChIP-qPCR (n = 3). c Chromatin compaction at the HIV 5´ LTR (n = 5). d Quantification of H3cit at the HIV-1 LTR in 1C10 cells under PMAi and GSK484 treatment using PLA-ZFP assay in 1C10 cells (n = 6). The box plots indicate median (middle line), 25th, 75th percentile (box), as well as minimum and maximum values (whiskers). e Quantification of Histone H3 at HIV-1 LTR in 1C10 cells under PMAi treatment using PLA-ZFP assay (n = 6). f Quantification of different H3cit residues with PLA-ZFP in 1C10 relative to H3 (n = 6 for H3cit2,8,17, n = 3 for the other antibodies). g CUT&Tag seq view of total H3cit after 24 h treatment over the HIV-1 LTR promoter. Gray line indicates TSS (HXB2 coordinate: 454). h Genome browser view of the H3cit signal over the TNFα promoter region. Gray line indicates the TSS. Data are shown as mean ± SEM. The number of independent experiments is denoted by n. Exact p-values were calculated with two-sided unpaired Student's t tests. Source data are provided as a Source Data file.