Fig. 5: De novo H3cit has a general effect on HIV-1 transcription, but not through Tat or acetylation.

a RT-qPCR quantification of short initiated (5´-LTR), early elongated unspliced (Gag), multiply spliced (Tat-Rev), and late elongated (GFP) transcripts in 5A8 treated with PMAi and GSK484. Exact p-values were calculated with two-sided paired Student's t tests (n = 5). b Levels of Tat at the HIV-1 promoter in 1C10 cells with PMAi and GSK484 using PLA-ZFP assay. The box plots indicate median (middle line), 25th, 75th percentile (box), as well as minimum and maximum values (whiskers). Exact p-values were calculated with two-sided paired Student's t tests (n = 5). c HIV-1 transcription in Tat negative J-Lat A72 and Tat positive A2 cells treated with PMAi and PADI inhibitors, measured by flow cytometry (n = 3). Exact p-values were calculated with two-sided paired Student's t tests. d 5A8 cells treated with PMAi and other latency reversal agents and GSK484 (n = 9 for PMAi, prostratin; n = 4 for JQ1, romidepsin, panabinostat). Exact p-values were calculated with two-sided paired Student's t tests. Data are shown as mean ± SEM. The number of independent experiments is denoted by n. Source data are provided as a Source Data file.