Fig. 6: Heterochromatin forms over the HIV-1 provirus in the presence of GSK484.
From: PADI4-mediated citrullination of histone H3 stimulates HIV-1 transcription
a H3K9me3 at the HIV-1 locus in 1C10 (ZFP) and 2C10 (ZFP-KRAB) cells measured by ChIP-qPCR. Exact p-values were calculated with two-sided ratio paired t-tests (n = 5 for 1C10, n = 3 for 2C10). b 1C10 and 2C10 cell HIV-1 transcription with PMAi and GSK484 treatment (n = 6). Exact p-values were calculated with two-sided unpaired t-tests. c Tat proximity to HIV-1 promoter in 1C10 and 2C10 cells with PMAi treatment (n = 6 1C10, n = 12 2C10). d H3K9me3 and HP1α levels at the LTR locus in 1C10 measured by ChIP-qPCR (n = 3). e HP1α proximity to the HIV-1 promoter in 1C10 and 2C10 cells with PMAi and PMAi and GSK484 treatment (n = 6). f GFP quantification by flow cytometry of 5A8 TASOR RNAi knockdown and 5A8 VPX overexpression cells (n = 3). Data is shown as mean ± SEM. The number of independent experiments is denoted by n. Exact p-values were calculated with two-sided paired Student's t tests unless otherwise indicated. Source data are provided as a Source Data file.
